Behavior of dicentric chromosomes in budding yeast
نویسندگان
چکیده
منابع مشابه
Resolution of dicentric chromosomes by Ty-mediated recombination in yeast.
We have integrated a plasmid containing a yeast centromere, CEN5, into the HIS4 region of chromosome III by transformation. Of the three transformant colonies examined, none contained a dicentric chromosome, but all contained a rearranged chromosome III. In one transformant, rearrangement occurred by homologous recombination between two Ty elements; one on the left arm and the other on the righ...
متن کاملMeiotic and mitotic behavior of dicentric chromosomes in Saccharomyces cerevisiae.
Meiotic recombination between a circular and a linear chromosome in Saccharomyces cerevisiae has been investigated. The circle was a haploid-viable derivative of chromosome III constructed by joining regions near the two chromosome ends via a recombinant DNA construction: (HMR/MAT-URA3-pBR322-MAT/HML) and was also deleted for MAL2 (which therefore uniquely marks a linear chromosome III). Recomb...
متن کاملThe role of replication bypass pathways in dicentric chromosome formation in budding yeast.
Gross chromosomal rearrangements (GCRs) are large scale changes to chromosome structure and can lead to human disease. We previously showed in Saccharomyces cerevisiae that nearby inverted repeat sequences (∼20-200 bp of homology, separated by ∼1-5 kb) frequently fuse to form unstable dicentric and acentric chromosomes. Here we analyzed inverted repeat fusion in mutants of three sets of genes. ...
متن کاملDicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast.
We have used mitotic spindle forces to examine the role of Sir2 and Ku in chromatin compaction. Escherichia coli lac operator DNA was placed between two centromeres on a conditional dicentric chromosome in budding yeast cells and made visible by expression of a lac repressor-green fluorescent fusion protein. Centromeres on the same chromatid of a dicentric chromosome attach to opposite poles ap...
متن کاملEvidence for cohesin sliding along budding yeast chromosomes
The ring-shaped cohesin complex is thought to topologically hold sister chromatids together from their synthesis in S phase until chromosome segregation in mitosis. How cohesin stably binds to chromosomes for extended periods, without impeding other chromosomal processes that also require access to the DNA, is poorly understood. Budding yeast cohesin is loaded onto DNA by the Scc2-Scc4 cohesin ...
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ژورنال
عنوان ژورنال: PLOS Genetics
سال: 2021
ISSN: 1553-7404
DOI: 10.1371/journal.pgen.1009442